Journal: Scientific Reports

Article Title: Macrophage polarization is associated with postoperative seroma development in breast cancer in the SerMa pilot cohort

doi: 10.1038/s41598-025-17139-2

Figure Lengend Snippet: CD68 and CD163 expression in patients without and with seroma formation. CD68 expression in the TME of a patient without ( A ) and with ( B ) seroma development and differences illustrated within a dot plot ( C ). CD163 expression in the TME of a patient without ( D ) and with ( E ) seroma development and differences illustrated within a dot plot ( F ). CD68 expression in the SAT of a patient without ( G ) and with ( H ) seroma development and differences illustrated within a dot plot ( I ). CD163 expression in the SAT of a patient without ( J ) and with ( K ) seroma development and differences illustrated within a dot plot ( L ). Scale bars represent 100 μm. The dot plots visualize the distribution of positive cells in patients without and with seroma development. The horizontal lines represent the median values. *p < 0.05. Correlation analysis of macrophages (CD68 + cells) counted in the TME and SAT ( M ) and Correlation analysis of CD163 + cells counted in the TME and SAT ( N ).

Article Snippet: Macrophages (CD68 + cells) were stained using an anti-human CD68 antibody (Monoclonal Rabbit IgG, Dilution 1:1000, Cell Signaling).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Macrophage polarization is associated with postoperative seroma development in breast cancer in the SerMa pilot cohort

doi: 10.1038/s41598-025-17139-2

Figure Lengend Snippet: Immunofluorescence co-staining of CD68, CD163 and PPARγ. Immunofluorescence: Expression of CD68 in red ( A ), CD163 in violet ( B ), PPARγ in green ( C ) and the overlay ( D ) in the TME of a seroma-developer at a magnification of 40 × lens and expression of CD68 in red ( E ), CD163 in violet ( F ), PPARγ in green ( G ) and the overlay ( H ) in the SAT of the same patient at a magnification of 40 × lens. The scale bar represents 100 μm. Cell-nuclei of all eukaryotic cells are stained in blue by DAPI to verify the cellular identity.

Article Snippet: Macrophages (CD68 + cells) were stained using an anti-human CD68 antibody (Monoclonal Rabbit IgG, Dilution 1:1000, Cell Signaling).

Techniques: Immunofluorescence, Staining, Expressing

Journal: Scientific Reports

Article Title: Macrophage polarization is associated with postoperative seroma development in breast cancer in the SerMa pilot cohort

doi: 10.1038/s41598-025-17139-2

Figure Lengend Snippet: Cell composition of seroma fluid measured by flow cytometry. Percentage of macrophages (M \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\varphi \text{, }\text{defined as CD68+ cells}$$\end{document} ) within the leukocyte population (CD45 + cells) ( A ). The median percentage of leukocytes was 7.7% (IQR = 4.0–18.8%). Percentage of CD163 + cells within the macrophage population ( B ). The median amount of CD163 + cells was 44.6% (IQR = 12.7–69.3%). The horizontal lines represent median values.

Article Snippet: Macrophages (CD68 + cells) were stained using an anti-human CD68 antibody (Monoclonal Rabbit IgG, Dilution 1:1000, Cell Signaling).

Techniques: Flow Cytometry